The 2021 membership of the Dunedin Youth Orchestra consists of the following players (in seating order):
| Concertmaster | *Rose Stevenson |
| Violin 1 | Victoria Lee |
| Skyla Murray | |
| Olive Ward | |
| Michelle Brown | |
| Romero Suggate | |
| Violin 2 | *Cindy Chou |
| Angela Fu | |
| Yuiko Yoshimura | |
| Savarna Yang | |
| Simone Mfumu-Nsuka | |
| Grace Hill | |
| Viola | *Cameron Monteath |
| Anjana Rathan | |
| Eve Gabor | |
| Robert Cresswell | |
| Cello | *Sophie Hamer |
| Ellen Walters | |
| Boudewijn Keenan | |
| Olivia Shakespeare | |
| Ella George | |
| Bass | Ariana Knudson Hollebon |
| Flute | Kim De Vantier |
| Em Jameson | |
| Kevin Chen | |
| Oboe | Melissa Van der Haegen |
| Callum Fotheringham | |
| Clarinet | *Sabine Grey |
| Ayla Biner-McGrath | |
| Josh Lewis | |
| Laura Canton | |
| Bassoon | Ella Cox |
| French Horn | Rita Close |
| Adair Clark | |
| Trumpet | *Serenity Hook |
| Quan Tay | |
| Trombone | *Finn McKinlay |
| Harp | Layla Sudol |
| Percussion | Ella George |
| Piano | Cathy Zeng |
Asterisks (*) indicate section principal. ‘+’ indicates guest players.